【摘要】 本文报道甲状腺微粒体抗体放射免疫(双抗体法)测定。并对技术指标作了简要探讨。应用本法测定100例正常人,x为10.3±2.4％(4.7～15.9％)。共检测167例各种甲状腺病患者。39例自身免疫性甲状腺炎中95％>15％,67例 Grave’s 中50.7％>15％,61例非自身免疫性甲状腺肿均<15％。本法与血凝法同时测定298例各种甲状腺病患者血清标本,二者的结果呈良好的相关性。根据实验结果并结合临床和病理,制定15％为甲状腺微粒体抗体放射免疫(双抗体法)测定正常和异常的临界值。更多还原

【Abstract】 A double-antibody RIA for anti-microsomal antibody in serum has been established in our laboratory soon after the purified microsomal antigen being avaiable to us. The antigen protein was radio-labeled ( 125I-Nal) by chloramine T. The relevant quality control indices were briefly discussed. The binding rate of 100 normal subjects assayed were x 10.3 ± 2.4% (4.7 - 15.9%), 95% were in between 5.5% and 15.1%. And that of 167 serum from the patients of thyroid diseases were:37/39(97%) of auto-immune thy-roiditis> 15%, 34/67 (42.2%) of Grave s dis. > 15%, and all the 61 non-autoimmune goiter were<15%. There is a good correlation, when concurrently tested with hemagg-lutiation method in 298 cases of various thyroid patients. After analysed the above data the author considered that the binding rate 15% is the suitable criterial value for normals and abnormals of our method.更多还原