【摘要】 本文采用 EA-花环法和YC-花环法分别对药物作用后的小鼠腹腔巨噬细胞表面Fc受体和 C3b受体进行体外观察。实验显示氢化可的松琥珀酸钠(HCSS)可明显抑制正常巨噬细胞表面的 Fc受体和 C3b受体。厌氧棒状杆菌菌苗激活的巨噬细胞,其 Fc 受体和 C3b受体的功能均较对照组活跃,并对 HCSS的体外抑制效应具有明显的对抗作用,表现为在 HCSS的体外抑制下,激活巨噬细胞的EA-花环率和YC-花环率均明显大于对照组(P<0.001)。实验还证明Neuro-tropin 对正常小鼠腹腔巨噬细胞膜受体无明显作用(P>0.05),但Neurotropin 作用后的巨噬细胞膜受体可拮抗 HCSS的体外抑制效应,Neurotropin处理后的巨噬细胞,经体外一定时间和一定浓度的HCSS作用,其EA-花环率和YC-花环率均高于未经Neurotropin预先处理的巨噬细胞(P<0.05,),提示 Neurotropin对巨噬细胞膜受体尚有一定的调理作用。本文采用的实验步骤为体外研究药物对巨噬细胞膜受体的影响提供简便而稳定的方法。更多还原

【Abstract】 The effect of immunomodulatory drugs on the Fc receptors (FcR) and C3b receptors (C3bR) of mouse peritoneal macrophages was investigated using in vitro EA-rosette and YC-rosette techniques. It showed that hydrocortisone sodium succinate (HCSS) had a significant suppressive action on the FcR and C3bR of normal macrophage. The FcR and C3bR functions of the C. parvum activated macrophages were greatly stimulated as compared with that of the control. Under the action of HCSS, the percentages of EA-rosette and YC-rosette of C. parvum activated macrophages were much higher than that of the unactivated ones (P<0.001). It could be concluded that the receptors’ function of the activated macrophages could antagonize the suppressive effect of HCSS It was also found that Neurotropin had no significant regulative action on the FcR and C3bR of normal macrophage (P>0.05), but could antagonize the in vitro suppressive action of HCSS The percentages of EA-rosette and YC-rosette on the Neurotropin treated macrophages were higher than that of ihe control (P<0.05) at given concentractions of HCSS This result showed that only under the immunological suppressive state, could Neurotropin modulate the function of the macrophage membrane receptors. The assay system adapted here has provided a simple and consistent method in investigating the action of drugs on the macrophage-membrane receptors in vitro.更多还原