【摘要】 本文使用TEMPO和脂肪酸氮氧自由基自旋标记脂质体和正常的与癌变的鼠肝细胞膜,研究了Vc对膜流动性的影响,探讨有关Vc抗肿瘤作用的机理。实验表明:Vc可以影响膜类脂和膜蛋白的运动,提高癌变细胞膜的流动性,并随Vc浓度的增加其作用速度加快,但对正常肝细胞膜流动性则无明显影响。此外,在一定浓度范围内,Vc对正常的与癌变的肝细胞膜的掺入能力有显著差异。更多还原

【Abstract】 The influence of Vc on membrane fluidity was studied in this artical, by using TEMPO and fatty acid nitroxide spin labeling liposome as -well as normal and malignant cell membrane of mouse liver, in order to explore the anti-cancer mechanism of Vc. The experiments show that Vc can influence the movement of membrane lipids and membrane proteins, increase the fluidity of malignant cell membrane, and the velocity of effect is speeded up with increasing Vc concentration. However, Vc has no evident influence on normal liver cell membrane’s fltiidity.In addition, within the studied concen-ration limits,the ability of Vc passing through membrane is obviously different between the normal and malignant cell of mouse liver.更多还原