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恶性疟原虫安徽分离株红内期的体外连续培养

THE CONTINUOUS CULTIVATION IN VITRO OF ERYTHROCYTIC STAGES OFI ANHUI ISOLATE OF PLASMODIUM FALC PARUM

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【作者】 韩范杨炳贵林珍陈锡慰张兆松赵慰先

【Author】 Han Fan et al.

【机构】 南京医学院寄生虫学教研室南京医学院寄生虫学教研室

【摘要】 本文报道自安徽省和县大包村采集人恶性疟现症病人和带虫者3倒虫血,脱纤维蛋白后,直接进行体外培养。虫体生长良好,到目前为止其中1例培养已达116天,另2例培养达95天,并有配子体出现。实验表明用于起始培养的虫血原虫密度较低仍可培养成功。

【Abstract】 The present paper deals with the estab-lishment of Anhui isolate of Plasmodi-um falciparum in continuous culture.Thereblood samples with malarial parasites,taken from a symptomatic malaria patientand two carriers in Dabao Village of HeCounty in Anhui province,were broughtto laboratory after defibrination and cul-tured directly.The parasites in culturehave been growing in normal multiplica-tion.Up to now,one of the three has beencultured continuously in vitro for 116 daysand the other two for 95 days.Gamet-ocyte formation could be observed in thecourse.The results show that even thoughthe level of initial parasitemia is ratherlow,erythrocytic stages of plasmodium fal-ciparum may be cultured in vitro success- fully.

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