【摘要】 外表健康鸭的粪便接种鸡胚,分离到一株具有血凝活性的病毒,暂称118毒株。病毒颗粒多数呈不规则圆形,直径80～130纳米,有囊膜,膜上有无数排列规则的纤突,其长度约为10纳米。将病毒接种鸡胚成纤维细胞培养可引起明显的细胞病变,特征为大部分细胞变圆,折光力增强,细胞质内有小颗粒积聚。本病毒对乙醚很敏感,50℃30分钟能将其灭活,MgCl₂对它无保护作用,对pH3敏感。5-碘-2′-脱氧尿核苷不能抑制其生长。本病毒不能溶解鸡的红细胞。根据本试验所得结果和血凝抑制试验,本病毒可能是一种目前国内新发现的正粘病毒。更多还原

【Abstract】 A haemagglutinating virus, designated strain 118, was isolated from the developing chick embryo inoculated with faeces of apparently healthy ducks.Most virions were irregularly round in shape, 80—130 nm in diameter, and possessed envelopes. Numerous spikes were regularly arranged on the periphery of the envelope, approximately 10 nm in length. When the virus was inoculated into the chick fibroblast monolayer, it caused distinct cytopathic effects. The majority of cells became round, more reflective, and small granules accumulated in the cytoplasm. The virus was ether-sensitive, acid （pH₃） -labile, inactivated at 50℃ for 30 minutes, and not stabilyzed by 5-iodo-2′-deoxyuridine. It could not haemolyse chick erythrocytes. According to its biological properties and haemagglutination-inhibition test, the isolate might be an orthomyxovirus,first discovered in China.更多还原