【摘要】 报道使兔感染日本血吸虫后,用含虫卵的肝脏切片作抗原的一种间接免疫酶染色法诊断日本血吸虫病的新方法。对86例确诊病人,124例健康成人和72例肺吸虫病人的血清进行检测,阳性率为98.8％,假阳性率仅0.8％。本法特异性强,敏感性高于COP及ELISA 法,且滴度与病人每克粪中排卵数呈正相关。因而,本法可用作考核防治效果的一个参数。更多还原

【Abstract】 Application of the indirect immunoperoxidase techique(IIP) in the diagnosis of patients with Schistosomasis Japonica is reported. Eggs in the tissue section of liver of the rabbits 45 days after infestation with S.Japonicum were used as antigen. All of the 86 but one serum samples from patients showed positive reactions at 1-100 or more dilutions, false positive rate was 0.8%. Cross reactions were not observed in the cases infestated with lung flukes.IIP was compared with ELISA and COP in patients with Schistosomiasis. IIP showed the highest sensitivity (p<0.05), and the titres of IIP was positively correlated with EPG. It is suggested that the reported procedure of the indirect immunoperoxidase technique is sensitive, and specific, and is useful as a tool for the diagnosis and investigation of Schistosomiasis japonica in endemic areas.更多还原