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猪胸腺素的生物活性——三种E花环形成试验测定的结果

BIOLOGICAL ACTIVITY OF PIC THYMOSIN DETERMINED WITH THREE KINDS OF ACTIVE E-ROSETTE FORMATION TEST

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【作者】 李曙光; 孙科柱; 燕美玉; 陈继嵩; 王长青; 易有年;

【Author】 Li Shuguang Sun Kezhu Yan Meiyu Cheng Chisung Wang Changqing Yi YounianDepartment of Immunology, The Central Laboratory of Hunan Medical College

【机构】 湖南医学院中心实验室免疫组; 湖南医学院中心实验室免疫组;

【摘要】 本文采用猪胸腺素作用于(1)猪胸腺淋巴细胞(PTLC)或人外周血淋巴细胞(PBL)、(2)经0.3%胰酶处理的人PBL、(3)经45℃加热30分钟处理的人PBL后形成EaRFC的三种方法,从EaRFC%平均增高率分析发现,用PTLC检测可反映出猪胸腺素的免疫活性。用人PBT和经胰酶或加热处理的人PBL时,虽多数情况EaRFC%平均增高率较高,但统计学分析却无显著差异。

【Abstract】 It is reported in this article that pig thymosin(made in Lou-yang洛阳) acted on 1. pig thymus lymphocytes (PTLC) or human peripheral blood lymphocytes (PBL), 2.human PBL treated by 0.3% pancreatin, 3- human PBL heated 30 minutes in 45℃ bath, and then an active E-rosette formation test (EaRFC) was made. The examination of PTLC could reflect the immune activity of pig thymosin from the analyses of EaRFC% (average increase rate and statistics). Even when the concentration of pig thymosin was as low as 0.0125 microgram, the capacity of EaRFC% was still increased. But when human PBL was used and treated with pancreatin or heated in 45℃ bath, under most conditions the average increase rate of EaRFC% was higher, but there was no significant difference statisticaly.

【关键词】 胸腺素; 玫瑰花结形成;
【Key words】 Thymosin Rosette formation;
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