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湖南省浏阳县卫氏并殖吸虫苹果酸脱氢酶同工酶(MDH)的初步检测

STUDY ON MALATE DEHYDROGENASE OF PARAGONIMUS WESTERMANI FOUND IN LIUYANG COUNTY, HUNAN PROVINCE

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【作者】 聂崇兴陈翠娥

【Author】 Nie Tsongxing Chen Cui-eDeportment of Parasitology, Hunan Medical College

【机构】 湖南医学院寄生虫学教研组湖南医学院寄生虫学教研组

【摘要】 本文报告对湘东地区浏阳县卫氏并殖吸虫不同发育阶段(囊蚴、后尾蚴、童虫和成虫)的MDH的检测结果,发现其主要特征酶带相一致,但酶带的数目和/或位置有差别。同时选择出以15只囊蚴为一组分作MDH检测,即可显现酶带;并检测到不同发育阶段的MDH酶谱与宿主(蟹)的MDH酶谱有明显差异。

【Abstract】 The malate dehydrogenase (MDH) pattern of paragonimus westermani collected from Liuyang, a county in the northeast of Hunan Province, was determined by PAGE. The characteristic patterns of this enzyme was similar in metacercaria, young worms and adult worms, but the locations and numbers of MDH bands showed a slight discrepancy. To demonstrate the MDH pattern, 15 metacercariae were needed at least. And it was found that the MDH pattern of the parasite in different developing stages was significantly different from that of their crab host.

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