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利用同位素15N对豆科作物固氮量测定的研究

Determination of nitrogen fixation of legume crops using isotope 15N

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【作者】 马昌燐温贤芳姚允寅周志祥刘晓兰

【Author】 Ma Changlin Wen Xianfang Yao YunyinZhou Zhixiang Liu Xiaolan(Institute for Application of Atomic Energy,Chinese Academy of Agricultural Sciences)

【机构】 中国农业科学院原子能利用研究所中国农业科学院原子能利用研究所

【摘要】 本文报导了利用同位素15N对豆科作物固氮量测定的研究。结果表明,15N-A值法和总氮差值法测定五个大豆品种和两种豆科牧草的固氮量值显著相关,两方法的测定结果明显地呈线性关系。通过比较非固氮作物重复间总氮测量值和15N%原子超测定值变异系数的大小,表明用AN值法估计固氮百分率比用总氮差值法可靠,试验结果还表明,AN值法测定固氮量比总氮差值法和乙炔还原法具有一定的优越性。

【Abstract】 Method for determination of N2 fixed by legume crops is reported. By difference method and 15N-A value method the amount of total N2 fixed and percentages of total N2 fixed by 5 soybeans and 2 species of legume grasses are highly corrected (r = 0.96, y = 1.051x + 0.037 for the amount of N2 fixation and r = 0.39, y = 0.245x +43.597 for the percentage of N2 fixation). It is shown that the estimated percentage of N2 fixed by AN- value method is more reliable than by difference method as a result of comparing the variation coefficents of total amount of N with the variation coefficents of 15N% atom excess for non-fixing N2 crops. It is found that the AN-value method has more advantageous than the difference method and the acetylene-ethylene method for measurement of dini-trogen fixation.

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