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贵阳地区老年人某些生化常数的测定

Deter mination of the Concentration of Six Substances in Blood of Old Persons in Guiyang District

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【作者】 戴安庆郭筱芬詹颖马小平贺晓惠

【Author】 Dai Anqing

【机构】 贵阳医学院生物化学教研室贵阳医学院生物化学教研室

【摘要】 <正> 老年医学在我国已日渐引起重视。过去在老年疾病的诊治中常用正常成人(包括老年及中青年)的生化指标作为判断标准,这不太符合老年患者的客观实际。为了探求老年人的实际生化常数,我们对贵阳地区67名60岁以上健康的退休职工进行了血浆中酸性磷酸酶、碱性磷酸酶、总胆固醇、钙离子、无机磷及乳酸脱氢酶同工酶的测定,同时对60岁以下健康成人的六项进行测定作为对照。

【Abstract】 The authors reported the blood level of acid and alkaline phospha- tase’, total cholesterol, calcium ion, inorganic posphorus and lactate dehy- drogenase (LDH) isoenzymes of 67 persons over sixty years old in Gui- yang district. The results were com- pared with the healthy adnlt controls under sixty years old (18-50). The plasma samples used for determlnation were anticoagulated with heparin. The acid and alkaline phos- phatase were determined in King Arm- strong method; the total cholesterol in modified Zak’s method; the calci- um ion in the EDTA-Calcon way; the inorganic phosphorus in method of Fiske-Subbarow. The lactate dehy- drogenase isoenzymes were separated by agarose gel electrophoresis, dyed in the Nitro-Tetrazolium Blue chloride (NBT) and determined with a densitometor. The resuIts indicated that the total cholesterol, calcium ion, LDH, of the old group wore all higher than that of the control (P<0.001 and P>0.002). But the LDH3 of the old group was lower than that of the control (P<0.002). None of the significant differences were observed in other item.

  • 【文献出处】 贵阳医学院学报 ,Journl of Guiyang Medical College , 编辑部邮箱 ,1984年03期
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