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脯氨酸含量在作物低温锻炼中的变化及同抗寒性的关系

CHANGES OF PROLINE CONTENT AT LOW TEMPERATURES IN RELATION TO COLO RESISTANCE

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【作者】 郭绍川刘玲玉

【Author】 Guo Shaoehuan (Department of Biology, Nei Monggol Teacher’s University)Liu Lingyu (Department of Biology, Baotou Teacher’s College)

【机构】 内蒙古师范大学包头师范专科学校

【摘要】 本文对抗寒能力不同的冬小麦、春小麦、莜麦及谷子的幼苗,在1,300勒克斯光强下,经12℃、5℃及-5℃的低温锻炼后,观测其幼苗中的脯氨酸变化与抗寒性之间的关系。在正常温度(25℃)及2,500勒克斯光强下,脯氨酸含量的差异与上述作物幼苗的抗寒力无相关性。在1,300勒克斯光强下,经12℃低温锻炼三天后,供试作物的脯氨酸含量均比在正常温度下生长的幼苗有提高,其增长的幅度与作物幼苗抗寒能力完全一致。抗寒最强的冬小麦增长近六倍,其次是春小麦,增加了三倍,而抗寒力差的莜麦和谷子则只增加50—75%。供试作物在12℃、5℃及-5℃温度下的脯氨酸含量均比在正常温度时的高,我们认为在12℃及5℃的增加是作物对低温的一种适应性反应,而-5℃时的增长则可能是受害的标志,是蛋白质降解的结果。脯氨酸积累的高峰在12℃时是第七天。本文对脯氨酸积累的阈温及对光的依赖性问题也进行了讨论。

【Abstract】 Hardened at low temperatures, free proline in the seedling of several crops (winter wheat, sprig wheat, naked oat and millet) was determined. There was not a correlation between proline and the crops’ cold resistance at the normal temperature (25℃).But, when hardened at various Iow temperatures (12℃, 5℉,-5℃), there was a clear correlation between the proline content of these crops’ seedlings and their cold resistance. The most suitable temperature was 12℃ and the maximum volume occurred on the seventh day at this temperature. No obvious threshold temperature of the proline accumulation was found. The increase of proline at-5℃ was the sign that the crops might have been damaged.

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