【摘要】 通过农杆菌（Agrobacterium tumefaciens）致瘤,从大豆属三个种的蛋白质含量不同的627份种质资源中,筛选出致瘤材料223份。其中蛋白质含量在43.00—45.52％的栽培大豆（Glycine max）品种4份;蛋白质含量在44.00—49.55％的半野生大豆（Glycine gracilis）类型23份;蛋白质含量在48.00—51.79％的野生大豆（Glycine soja）类型19份。通过组织培养,从瘤组织中得到了脱菌的愈伤组织。生化检测表明,3个种的大豆瘤来源的部分愈伤组织含有T—DNA。并通过液体培养,建立了含T—DNA的细胞系。现已培养50多代,胭脂碱合成酶基因仍然稳定地整合在大豆基因组中,其染色体数为2n=40,表明是含T—DNA的稳定的细胞系。为基因转移打下了良好的基础。 T₁质粒作为植物基因工程的载体受到了广泛的重视,它的载体功能是通过擦伤感染致瘤的过程进入植物细胞来实现的,因此,通过致瘤反应筛选出理想的受体显得非常重要。我们对大豆属的致瘤反应曾作过一些报道,为了充分利用我国大豆的丰富资源,选育出高蛋白品种,我们进行了基因转移的高蛋白受体系统的研究,本文报道这些研究的初步结果。更多还原

【Abstract】 223 genotypes which can form tumors with T-DNA have been screened from 627 genotypes, of three species of Glycine with different protein content, by infection with strains of Agrobacterium tumefaciens. Among 223 genotypes, 4 of cultivated soybean (G. max), 23 of semi-wild soybean (G. gracilis) and 19 of wild soybean (G. soja) contain protein at 43.00-45.52%, 44.00-49.55% and 48.00-51.79% respecti-vely. The bacteria free calli were obtained from tumor tissues by means of tissue culture. Biochemical detection showed that partial calli derived from tumor tissues of three species mentioned above contain T-DNA of Ti plasmid. Cell lines of the calli with T DNA have been established by using liquid culture. Up to present, 50 generations of the cell lines have been propagated and the nopaline synthetase gene is integrated stably in the genomes of soybeans. Chromosome number of the cell lines is normal, 2n=40, basically. This demonstrated that these cell lines are stable ones containing T-DNA. This is a good beginning for transferring high protein gene by using Ti plasmid.更多还原