【摘要】 本文研究了 Os-732细胞株的超微结构及酶的特性。在 SEM 下细胞形态呈多样性,细胞表面形成树枝状伪足;TEM 下胞质内含丰富的扩张粗面内质网,发达的高尔基复合体及分泌泡;大量碱性磷酸酶作用的沉积物出现于胞膜表面。这些特征均显示它与成骨细胞的相似性。为证明此株细胞是威骨系统的肿瘤提供可靠的依据。结合细胞株的动物接种,染色体和免疫学特性等研究,证明此细胞株是一新的成骨肉瘤细胞株。更多还原

【Abstract】 The ultrastructural and cyrochemical characteristics of Cell Line Os-732,establi- shed from a human osteosarcoma,are reported in this paper. Scanning electron microscopy studies showed that the cells were irregular in shape and the cell surface was covered with large number of microvilli.Sometimes,a few of ziotic blebs were observed.It seemed that the cells are attached to the glass by lameli- podia and filopodia of variable lengths. Transmision electron microscopy studies showed that the cells had large nuclei with prominent nucleoli.Their cytoplasm contained abundant free ribosomes,prominent Golgi complexes,a few mitochondia and scattered dense lysosome-like bodies.The rou- gh endoplasmic reticulum was often expanded into many vacuoles,filled with modera- tely dense amorphous meterials.No calcuium apatite deposits and virus particles were observed. The ultrastractural localization of phosphatase activity studies demenstrated that most of the cells showed deposits of reaction products of alkaline phosphatase on the whole circumference of reaction products of alkaline phosphatase on the whole circum- ference of sectioned plasma membrane or as scattered foci.Sometimes,deposits of rea- ction products were also observed on the nuclear membrane.No deposition of reaction products of acid phosphatase was observed except within some lysosomes.更多还原