【摘要】 <正> 新化学物质的生产和应用不断增加,人类环境中化学物质致突变、致癌方面的研究已引起很大注意,并发展了不少短期快速筛选方法,黑腹果蝇(Drosophila melanogaster)遗传试验是其中之一。果蝇属真核细胞生物,更多还原

【Abstract】 A number of test systems using drosophila melanogaster for detection of mutagens andpotential carcinogens in human environment have been developed. The important advantagein the use of this fly is its short generation time(10-12 days), the relatively simple facilityneeded for its breeding and the readiness in choice of manner of exposure, i. e. satisfac-tory routes of administration. The additional advantage apperas to be its capacity of metabo-lising drugs comparable to those found in mammals. In this paper we are going to introduce the genetic basis aPd tbe practical laboratorytechnics of the most useful SLRL test. Our investigations were carried out with the knownmutagen MMS, using DMSO as the negative control. The SLRL frequencies for MMSwere 5.2%in 1.0 mM and 14.1% in 2.0mM respectively. There is no indication of arecessive lethal in the negative controls after having tested up to 596 chromosomes. These results along with the available data in recent literatures suggest that the SLRLtest is among the generally accepted simple and objective screening systems helpful to thepromotion of the study on chemical mutagens and potential carcinogens.更多还原