【摘要】 <正> 近几年来,由于鲎试验不能证明伤寒患者血浆中存在内毒素,故对伤寒发病的内毒素刺激学说发生了怀疑;同时,内毒素类似抗原抗体复合物对人类白细胞的作用和免疫复合物引起伤寒肾脏并发症问题,益受重视.本文测定31例伤寒患者血清免疫复合物（IC）及补体C₃,并讨论其意义.材料与方法（一）血清来源采自31例伤寒患者,其中普通型30例.感染性休克死亡1例;男17例,女14例.（二）测定方法IC测定用酶联免疫吸附试验（ELISA）,操作步骤按文献2方法,用EDTA分段沉淀法提取人血清中的C_1q,选择适量C_1q,包被微量反应板,加稀释待检血清孵育后,加辣根过氧化物酶,标记马抗人IgG,以邻苯二胺为底物,在72型分光光度计的492nm处测OD,后者0.3以上判为阳性.30例正常更多还原

【Abstract】 Determinations of serum IC and complenent C₃ were carried out on 31 patients suffering from typhoid fever. Specimens were taken once during the febrile period and again when the fever subsided. The serum IC levels of 33% of the cases were above the normal range. During the febrile period, the serum C₃ levels increased above normal range, but returned to normal levels as fever subsided. If the C₃ levels are below the normal range, the possibility of septic shock should be borne in mind.更多还原