【摘要】 将毛白杨、哈密瓜、大豆和烟草愈伤组织分别接种在附加 4mg IAA/l 和0.5mg KT/l 的Miller 固体培养基上。于接种后的0,5,10,15,25天分别取样测定 IAA 氧化酶和过氧化物酶的活性,发现酶活性均以哈密瓜愈伤组织的为最高,烟草愈伤组织的最低;酶活性在第10天时达到高峰。但在整个培养期中,四种愈伤组织的过氧化物酶的同工酶谱没有明显变化。仅哈密瓜愈伤组织的 IAA 氧化酶同工酶10个酶带中的 A₆、A₇、A₈三条酶带活性比其它三种愈伤组织中的相应酶带活性高出3—17倍。更多还原

【Abstract】 Aspen,Hami melon,soybean and tobacco calli were incubated in Miller’s solid medium supplemented with IAA 4mg/l and kinetin 0.5 mg/l.Activities of IAA-oxidase and peroxi- dase were determined at 0,5,10,15 and 25 days after incubation.The activities of IAA oxi- dase and peroxidase of Hami melon callus were found to be the highest and the tobacco was the lowest among the four different kinds of calli,both enzymes showed their peak value in 10 days after incubation. There were no change in pattern of peroxidase isoenzyme among the four kinds of calli during the incubation,but the activities of IAA-oxidase and its isoenzyme of Hami me- lon at bands A₆,A7 and A₈ were 3 to 17 folds higher than that of corresponding isoenzyme of other three kinds of calli.更多还原