【摘要】 链霉菌S-81-24所产生的糜蛋白酶抑制剂,其高峰期是在摇瓶培养的2～3天。提取时既可以利用大孔树脂吸附也可以用正丁醇在pH2～3进行萃取。它易溶于水、甲醇,但不溶于乙酸乙醋、乙醚、苯和氯仿等非极性有机溶剂。没有明显的紫外吸收峰。初步测定,它不仅对糜蛋白酶有强的抑制作用而且对胰蛋白酶、胃蛋白酶也有一定的抑制作用。但是这种多功能性是来自样品的同一组分还是不同组分尚待今后样品进一步纯化后再行深入探讨。更多还原

【Abstract】 The production of the chymotrypsin inhibitor by Streptomyces S-81-24 reached a maximum after two to three days in submerged culture. Chymotrypsin inhibitor can be extracted either by absorption using D-resin or with n-butanol at pH 2~3. It is soluble in water and methanol, but insoluble in non-polar solvents, such as, ethyl-acetate, ether, benzene, chloroform etc. Novisible absorbtion is observed in U. V. spectrum. It inhibits not only chymotrypsin strongly but also inhibits trypsin and pepsin to a certain extent. Whether this poly-functional action is due to the inhibitor as a whole or due to the different components of the substance needs further investiga-tion.更多还原