【摘要】 本文比较了日本血吸虫肺期童虫在甲、乙两种培养基中的存活情况、生长速度和发育情况,表明乙培养基较甲培养基为宜。童虫在乙培养基中可存活30天,达生殖器官发育期,其大小为培养零天时的童虫大小的5倍。表明本试验所建立的童虫体外培养方法简单易行,可用于血吸虫免疫学、生理生化学和抗血吸虫药物筛选等研究,并提出了评价肺期童虫体外培养效果的标准。更多还原

【Abstract】 This paper deals with some features of growth and development of three-day-old schistosomula of Schistosoma japonicum recovered from lungs of experimentally infected mice in both medium A and medium B. In young schistosomula, pigment appeared on the third day and cecal conjunction began on day 12. The degree of their development reached stage 3 (caeca united) by day 15. However, it occurred somewhat later as compared with development in vivo. About 50% of the schistosomula cultured in medium B can survive for at least 30 days and their size is five times as big as that at the beginning and also the optimal development reached stage 4 (organogeny).The in vitro procedures presented here for cultivating schistosomula might be used for the screening of antischistosomal drugs as well as for immunological, physiological and biochemical studies of Schistosoma japonicum.Finally, criteria are proposed to evaluate the effect of the in vitro cultivation of lung-phase schistosomula.更多还原