【摘要】 喹吡酮对体外培养的姜片虫有较强的作用,接触药液3.9μg/ml1′内腹吸盘即迅速松弛扩大。虫体的反应与药物剂量有关。剂量在250μg/ml以上时,虫体明显挛缩。经低浓度药物(3.9μg/ml和15.6μg/ml)作用3′或10′的虫可于30′内恢复其体态和活动,并在24、48、72小时常规培养中存活和产卵。药物浓度在62.5μg/ml以上时,虫的体表出现泡状隆起,在以后的常规培养中排卵量显著减少,24小时内全部死亡。但从培养液中收集的虫卵,于30℃恒温培育12～15天仍可孵出毛蚴。 接触吡喹酮3′或10′的姜片虫,其体壁、肠道和生殖器官均出现不同程度的退行性变化。更多还原

【Abstract】 Pyquiton has a strong effect on Fasciolopsis buski in vitro. Ventral suckers of the affected worms become flaccid and dilated within 1 minute after contacting with drug solutions. The response of the worm body depends on the dosage level: in small doses, the main feature is extension and elongation, while in large doses, the response appears earlier and persisted shorter in duration, followed by the contraction of the body, which is the main response when the dose is over 250μg/ml. After exposing to small doses of drug solution (3.9μg/ml and 15.6μg/ml) for 3 or 10 minutes, the affected worms recover their natural morphology and motility in 30 minutes and oviposit under the routine culture conditions for 24, 48 and 72 hours. When the dose is over 125μg/ml, bubble-like elevations appear on the body surface of the worms, oviposition is greatly reduced and all the worms die at the end of 24 hours’ cultivation. Eggs collected from the cultivations hatched out at 30℃ 12-15 days later.Histologically degenerative changes of various extent were seen in tegument, intestine, testes, ovaries of the worms under the light microscope.更多还原