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国外引进的布鲁氏菌噬菌体增殖试验观察
The Experimental Observation on Propagation of Brucella——phages from CVL.Wegbridge
【摘要】 <正> 布鲁氏菌噬菌体的研究,自Gwatkin试图从患布氏菌病牛的病原材料中分离出噬菌体以来已有50多年的历史。但是,只有苏联在五十年代分离的Tb噬菌体为国际所公认,是可用于布氏菌鉴定的噬菌体。其他方面的工作多停留在噬菌体的分离、裂解活性的测定上,而且所分离到的噬菌体活性低、不稳定,难以传代保存。自六十年代末由于试验技术的统
【Abstract】 A Preliminary study of the characteristics of 12 strains of Brucella-phagewas undertaken with special reference to their PFU, RTD and to plaque morph-ology in 5 of them.The results showed that PFU from 7 host (Brucella Spp.)strains were 109-11whereas from another 5 were 106-8.Our experiments demonstra-ted that RTD of 3 strains was found to be 10-4, 6 strains 10-2, and 3 strains10-1.The plaque morphology of 5 phages were compared after an incubation of18h.They varied greatly with different phage strains when adapted to differenthost species.A large and clear plaque was revealed in R/O strains, and that ofBK2 appeared quite small, and sometimes difficult to be distinguished.
- 【文献出处】 中国地方病学杂志 ,Chinese Journal of Endemiology , 编辑部邮箱 ,1983年02期
- 【被引频次】3
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