【摘要】 用已长出地面的风信子(Hyacinthus orientalis L.)花序为试验材料。将花序分切成不同的部分,分别接种在附加不同量的NAA及BA的MS琼脂培养基上。在无激素的培养基上,除花序轴切段能形成极小的鳞茎原基以外,其余各种外植体都不能分化形成鳞茎原基。培养基中只加NAA或BA也不易分化生成小鳞茎。培养基中同时加入NAA及BA时,如NAA的用量较BA高得较多时,虽能很快形成愈伤组织,但也不易分化生成小鳞茎。如NAA的量小于BA,则花序不同部分的外植体都可形成大小不等的小鳞茎;但花被、子房较花序轴切片及花柄需要较高的激素用量。多年来,在生产上,风信子(Hyacinthus orientalis L.)是用从鳞茎上生出的小鳞茎,或是将基盘挖掉使鳞片上长出小鳞茎的方法繁殖。近年来,有些人用组织培养的方法,用鳞片或花序切段为外值体研究风信子的繁殖〔1—3〕。因风信子多种植在土中,用鳞片进行组织培养,灭菌较困难:而用长出地面的花序为材料不仅灭菌较容易,此外,原鳞茎还可保存下来,有利于以后的工作。本工作就是研究激素对花序的不同部分的分化形成小鳞茎的影响。更多还原

【Abstract】 Inflorescences of hyacinth after emerged from the soil surface at different height were used. Cut the inflorescences into different parts: peduncle, rachis, pedicel and flower bud. Then cut them into suitable pieces. In vitro culture, the effect of hormones on the regeneration of bulblets on the inflorescence explants was investigated. When the culture medium was basal medium without hormones, only small bulblet primordia(about 1 mm in length) regenerated at the basal end of the rachis segments. If NAA or BA added to the medium separatily, the bulblets were rarely regenerated. When NAA and BA added together to the basal medium and the amount of NAA is less than that of BA, the bulblets are differentiated from explants. The explants of perianth and ovary require higher amount of auxins than that of rachis, pedicel and peduncle.更多还原