【摘要】 仅有少量文献指出,豚鼠T淋巴细胞的检测可用与免红细胞形成E花结的方法,至于酯酶染色在豚鼠T淋巴细胞检测中的意义则尚未见诸报道。鉴于后一方法简便,且不仅适用于外周血,亦适用于组织切片中T淋巴细胞的检测。为此我们分离豚鼠外周血,用两种方法进行了对比观察。实验中采用12只豚鼠,结果如下: 1、豚鼠外周血淋巴细胞的E花结形成率为34.3±8.5％,与文献中报道近似。 2、豚鼠外周血淋巴细胞中,89.1±7.3%呈ANAE(+)。 3、ANAE(+)淋巴细胞中,只39.3±10.2％形成E花结;而E花结形成细胞几全部(93.7％)为ANAE(+)。豚鼠T淋巴细胞究应以与兔红细胞形成E花结,抑以ANAE活性作为检测指标,认为应进一步查明。更多还原

【Abstract】 Only a few reports on guinea pig T lymphocyte derection with rab bit RBC(RRBC)rosette formation have been presented.But there was no information about the significance of esterase staining in this field. Since the simplicity and facility of the esterase staining and it can be used both for the peripheral blood and tissue sections, we studied this method in comparison with the RRBC rosette formation. 12 guihea pigs’ perpheral b1ood were studied,the resuIts are as follows 1、E rosette forming lymphocytes were 3 4.3±8.5％.It was thesatlae as previotis reports. 2、ANAE(+)lymphocytes were 89.1±7.3％. 3、Only 39.3±10.2％ of ANAE(+) lymphocytes showed E rosette formation,bat almost all(93.7％)of ERFC were ANAE(+). Whether the RRBC rosette formation or the ANAE activity may be selyed as the marker of guinea pig T lymphOCyte should be studied fur ther.更多还原