【摘要】 两个品种的荔枝花药,接种在MS附加KT2mg/1、2,4-D2mg/1、NAA0.5mg/1培养基上培养,经过70—90天即诱导出愈伤组织;再以MS和改良B₅附加KT0.5mg/1+NAA0.1mg/1+蜂皇浆400mg/1+LH500mg/1培养基进行分化培养,经30天左右分化出胚状体,再转入MS和改良B₅培养基附加蜂皇浆400mg/1、谷氨酰胺500mg/1、LH500mg/1进一步培养出芽;最后用1/2改良B₅或2.5倍White的大量元素附加KT0.1mg/1、IAA0.01mg/1、LH500mg/1、谷氨酰胺1600mg/1进一步分化成具有根、茎、叶器官的完整植株。目前已获得小苗24株,其中4株系具有正常根、茎、叶的完整小植株。根尖细胞压片检查结果,染色体数为2n=15,证明它们是单倍体植株。更多还原

【Abstract】 A series of experiments have been carried out on inducing pollen plants of two bestvarieties of litchi tree----Gushan Zfcaoher (wildng nudet) ’and Putain Chentze (old purple)since 1980.Anthers were inoculated on the MS medium added with KT 2mg/l,2,4-D 2mg/l,NAA 0.5mg/l,3% sucrose and 0.7% agar.After 70-90 days of culture when the calli formed from the anthers,they were transferred to the second medium,which is MS of modified 85 medium containing KT 0.5mg/l,NAA O.lmg/1,bee royal jelly 400mg/l,LH 500mg/l,3% sucrose and 0.7% ager fcr differentiation of the embryoids.About 30 days later,hundreds of embryoids formed.These embryoids were transferred to the MS or modified 85 medium containing bee royal jelly 400mg/l,L-glutamine 500mg/l and 3% sucrose.The young shoots grew up from some cf the embryoids,and finally were transferredto the - modified 85 medium or white medium (2.5 times of nutrients) added with KT 20.1mg/1,IAA 0.01mg/1,LH 500mg/l,L-glvtamine 1600mg/l,1% sucrose and 0.7% agar.In these conditions the intact plantlets were established,and then had roots,stems and true leaves.24 plantlets were obtained among them four intact plantlets were growing well and normally.Cytological observations of root tip cells of the plantlets showed that the mitotic metaphases of root tip cell had 15 chromosomes.So they were haploid plantlets of Litchi chinensis.更多还原