节点文献
应用纯化血凝抗原(冻干)诊断耕牛日本血吸虫病的研究
DIAGNOSIS OF SCHISTSOMA JAPONICA INFECTION IN CATTLE BY AN INDIRECT HAEMAGGLUTINATION TEST
【摘要】 应用理化及免疫吸附方法对血吸虫虫卵抗原进行了纯化,然后致敏绵羊红血球,制成冻干的血凝抗原,先后对数百头感染血吸虫牛和未感染血吸虫牛进行了血凝试验,试验结果表明有较高的敏感性、特异性和较好的重复性,对感染血吸虫牛的检率可达96.4%,假阳性很低;此外还对寄生血吸虫牛和牛治愈后血液内抗体消长情况作了测定。
【Abstract】 An indirect haemagglutination test has been developed for the detection of Schistosoma japonica antibody.Tanned glutaraldehyde fixed sheep erythrocytes were sensitized with the soluble egg antigen which had been purified with physical and chemical methods or the method of immunoabsorption.Hundreds of schisto some-free cattle and buffaloes and the animals infected S.japonica have been tested.The test have been found to have good reproducibility,high sensitivity and specificity.94.6% of S.japonica infected cattle were positive while there was little cross- reaction.The variation of the quantity of the antibody in the infected cattle and in the cured cattle has also been observed
- 【文献出处】 畜牧兽医学报 ,Chinese Journal of Animal and Veterinary Sciences , 编辑部邮箱 ,1983年01期
- 【被引频次】4
- 【下载频次】18