【摘要】 红曲霉（Monascus sp.）的葡萄糖淀粉酶具有多型性。比较了原始菌株AS3.978及其变异株AS3.2199及AS3.3491所产生的葡萄糖淀粉酶的凝胶电泳图谱。证实了三者所产生的主要区带E₃和E₄是互相对应的。研究了AS3.3491在不同的培养条件下,一些酶的生成与变化情况。发现在葡萄糖淀粉酶形成的同时,有酸性蛋白酶、α-淀粉酶和α-半乳糖苷酶的生成,没有发现有中性蛋白酶、碱性蛋白酶、β-半乳糖苷酶、甘露糖苷酶和甘露聚糖酶。培养时加入酸性蛋白酶抑制剂并不影响葡萄糖淀粉酶多型性的模式。更多还原

【Abstract】 The glucoamylase from Monascus sp. exists in multiple molecular forms. The comparison of the patterns of disc gel electro phoresis of the original strain AS 3.978 and the mutant strains AS 3.2199 and AS 3.3491 indicated that the main two bands E, and E₄ produced by them were corresponding to each other.Investigation of the formation and changes of some enzymes under different cultural conditions showed that accompaning to the glucoamylase formation acidic proteinase, α-amylase and α-galactosidase were produced, while neutral proteinase, basic proteinase, β-galactosidase, mannosidase and mannase were not found. The existence of acidic proteinase did not affect the pattern of the multiple molecular forms of glucoamylase.更多还原