节点文献
特异绒毛膜促性腺激素抗血清的制备及放射免疫测定
PREPARATIONS OF SPECIFIC ANTISERA TO hCG AND DEVELOPMENT OF hCG SPECIFIC RADIOIMMUNOASSAY
【摘要】 本文报道用小剂量完整的特异绒毛膜促性腺激素(hCG-β)作为免疫原,经过较长时间的免疫产生一系列抗血清,筛选后得到一个高滴度、高亲和力、特异性好的hCG-β抗血清,并用它建立了特异的hCG 放射免疫测定法。经600个不同样品的测定,结果证明该方法能在正常生理水平下(LH 存在下)特异地测定hCG,灵敏度较hCG/LH 放射免疫测定法提高10倍以上,为10毫国际单位/毫升(lst IRP hCG)。
【Abstract】 It is here reported that a series of hCG-specific antisera have been produceed,usingsmall doses intact subunit of hCG as an antigen,and a specific radioimmunoassay(RIA)for hCgG determination aftrer long term immunizion has been established.Results onmore than 600 different samples have shoun that the RIA can recongnize hCG at normalphysiological level of LH,and the sensitivily of this assay was ten times more than theconventional hCG/LH RIA,i.e.10 mI.U./ml(lst IRP hCG).
【关键词】 hCG;
Specific antisera;
Radioimmunoassay;
【Key words】 hCG; Specific antisera; Radioimmunoassay;
【Key words】 hCG; Specific antisera; Radioimmunoassay;
- 【文献出处】 生殖与避孕 ,Reproduction & Contraception , 编辑部邮箱 ,1983年04期
- 【被引频次】7
- 【下载频次】43