【摘要】 <正> 早在1956年前我们曾对松树和柞树的外生菌根真菌进行了分离。当时获得3株菌根真菌的纯培养,经初步鉴定属牛肝菌属(Boletus)、乳菇属(Lactarius)和滑菇属(Hebeloma)。对油松(Pinus tabulaeformis)幼苗回接试验取得了成功。在樟子松(Pimus sylvestris)幼苗的接种试验中,也获得了较好的结果;菌根化促进了樟子松幼苗的成活(张宪武等,1964)。 1978年以来,我们对外生菌根真菌又进行了研究。从不同林分下采集到能形成菌根的真菌子实体,在无菌条件下用切割组织法以分离菌根真菌,获得了纯培养菌株,根据子实体的特征进行了鉴定(邓叔群,1963)。同时对培养基选择及幼苗接种效果也进行了试验。本文是我们研究的初步结果。更多还原

【Abstract】 In 1978-1980, 30 fruit-bodies of fungi were collected from 4 species of pine trees (Pinus massoniana, P. tablaeformis, P. koraieusis, P. elliottii) in north-eastern and southern provinces of China. By using sterile methods 13 pure fungi cultures (Pisolithns tinctorius, Suillus granulatus, S. bovinus, Boletinus cavipes, B. plctus, Gomphidius sp., Armellaria mellea, Lepiota procera, Russula delica etc.) were obtained from the mycorrhizal fungi basidiospores collected from 30 fruit bodies. 10 cultuted media were used. It denoted that the best medium for fungi development was the one which contained the extract of potato malt extract dextrose and agar (P.D.M.A).Four species seedlings of pine (Pinus massoniana, P. tablaeformis, P. sylvestris, and Larix gmelini) were infected by the 13 pure fungi cultures . These seedlings grew promptly, especially when they were infected by Suillus bovinus, Boletinus cavipes, Cortinarius sp..更多还原