【摘要】 用分子筛凝胶（Sepharose 2B）柱层析法,分别从小鼠SRS腹水上清液和瘤细胞中分离提取了SRS腹水瘤病毒（SRSV）。经电子显微镜观察表明,腹水上清液中的SRSV属于C型,瘤细胞中的SRSV主要属于A型。利用病毒内源性反应,对分离提取的SRSV进行了分析鉴定。首先用非离子去垢剂对病毒进行破壳处理,反应需要四种脱氧核苷三磷酸作底物,二价阳离子(Mg²⁺,Mn²⁺)作为酶的激活剂,而Ca²⁺却无作用。一价阳离子有一定促进作用。核糖核酸酶（RNase）对反应有明显的抑制作用,而脱氧核糖核酸酶（DNase）则无抑制作用;低浓度放线菌素D对反应无明显抑制作用,高浓度则有部分抑制作用。实验结果证明从腹水上清液和瘤细胞中所分离提取的SRSV属RNA肿瘤病毒。更多还原

【Abstract】 The SRS virus of the T-S-Z system was isolated from the supernatant and tumor cells of the ascites of the SRS mouse by sepharose 2B gel chromatography respectively. Electronmicroscopic observation of the isolated SRS virus indicated that the SRS virus in the supernatant was attributed to type 0 RNA tumor virus and in tumor cells, type A RNA virus. The study of the endogenous reaction indicated that virion should be first disrupted with Triton-X 100 or NP-40. Divalent cations such as Mn2+, Mg2+ were required absolutely for the reaction. All four deoxyribonucleoside triphosphates were required, and if one or more of the deoxyribonucleoside triphosphates was omitted, no reaction activity could be detected. Reverse transcriptase activity was inhibited significantly, while the RNase was added in the endogenous reaction. However, no inhibition was observed with DNase, The endogenous reaction was partially inhibited at the higher concentration of actinomycin D, while no inhibition was observed at lower concentration.The experimental results mentioned above indicated that SRS virus was identified as oncornavirus.更多还原