【摘要】 我们对中药清热药(知母、生地)的作用机理和知母的有效成分菝葜皂甙元抑制人红细胞钠、钾泵的特性进行了研究。结果表明菝葜皂甙元和生地梓醇对人红细胞钠、钾泵具有显著的抑制作用,提示清热药的清热作用可能与钠、钾泵被抑制有关。 菝葜皂甙元对人红细胞钠、钾泵的抑制作用是可逆的,抑制作用的发生是比较缓慢的,并可被细胞外Na~+所增强,而被细胞外K~+所拮抗。菝葜皂甙元对红细胞摄取Rb~+的抑制属混合性抑制。菝葜皂甙元掺入重新封闭的人红细胞影泡的研究,提示菝葜皂甙元抑制红细胞钠、钾泵的作用部位可能在细胞膜的外侧面。更多还原

【Abstract】 ZHI MU (知母 Anemarrhena asphodeloides Bge) is a Chinese herb used as an antipyretic agent in traditional Chinese medicine. It is more effective for the patients with "internal heat" (内热)due to deficiency of YIN(阴). Sarsa-sapogenin, an effective component extracted from ZHI MU, has been demonstrated to be an inhibitor of Na+, K+-ATPase.The mechanism of its antipyretic action was investigated. And the characteristics of inhibition of the sodium-potassium pump by sarsa-sapogenin in human red cells were studied.The results indicated that sarsa-sapogenin can inhibit the sodium-potassium pump or Na+, K+-ATPase of human red cells, and SHENQ DI (生地 Radix Rehmanniae) catalpol can also inhibit the sodium-potassium pump in human red cells. The results suggested that the effects of reducing intense internal heat of antipyretics may be related to the inhibition of the sodium-potassium pump.The effect of sarsa-sapogenin inhibition on the sodium-potassium pump in human red cells is reversible and stable. The development of sarsa-sapogenin inhibition on the sodium-potassium pump is slow. Its inhibition is favored by external sodium and is antagonized by external rubidium. Sarsa-sapogenin is a mixed inhibitor to external rubidium in human red cells.The studies of sarsa-sapogenin incorporating into the regealed ghosts suggested that the acting site of its inhibition on the sodium-potassium pump in human red cells may be at the outside of the cell membrane.更多还原