【摘要】 本文报道血样经醋酸乙脂提取、分离丹参素后,用荧光光谱法加以测定。丹参素醋酸乙脂液激发光谱与荧光发射光谱峰的波长各为λ_Εχ285nm,λ_Εχ314nm;浓度在1.0～9.0×10^-6g/ml,范围与荧光强度呈线性关系;溶液的荧光强度稳定。由本法测得血浆中的丹参素回收率平均为76.47士2.427％（CV=3.174％,n=20）;血药浓度-时间（1gc-t）曲线表明,丹参素的药物动力学为单室模型,其消除速率常数和生物半衰期为:Kel=0.04561士0.0141(min^-1);t（1/2）=16.58士5.768（min）。更多还原

【Abstract】 A spectrofluorometric procedure for the determination of rabbit plasma level of "DAN-SHEN-SU" [D( + )×-3, 4-dihydroxyphenyl lactic acid] was proposed, and ita phamacokinetic parameters were obtained from the corresponding plasma level-time curve measured.In ethyl acetate medium, "DAN-SHEN-SU" showed an emission maximum at 314 nm (the excitation maximum was at 285 nm) and its concentration ranging form 1.0 to 9.0 × 10-6g/ml presented a linear relation with the fluorescent intensity which remained stable for three hours, correlation coefficient, x=0.9996; regression line, 0= 1.0168 ±10-7F-1. 815 ±10-7.The data of fluorescent intensity of"DAN-SHEN-SU"extracted with ethyl acetate from rabbit plasma within the above concentration range were also quite satisfactory, r=0.9997; C = 1.497±10-7F-1.470±10-7.The average recovery of "DAN-SHEN-SU" from plasma was 76.47 ±2.427%, coefficient of variation, 0. V. = 3.147% (n = 20).According to the existing experimental conditions, the rabbit plasma levels of "DAN-SHEN-SU" administered intravenously were measured. The resultant graph of log plasma concentration-time plotted was a straight line, suggesting that the pharmacokinetics of "DAN-SHEN-SU" was a one-compartment open body model with the following parameters: elimination rate constant, Kel = 0.04561 ±0.0141 (min-1); biological half life time t1±2 = 16.58±5.768(min).更多还原