【摘要】 本文报道用酶联免疫吸附测定法(ELISA)检测水稻普通矮缩病传毒昆虫带毒率。用戊二醛二步法制备辣根过氧化物酶与抗血清丙种球蛋白的结合物,用ELISA法检测水稻普通矮缩病传毒昆虫——黑尾叶蝉388头,测定结果与常规生物接种测定比较,其符合率为92.7%。更多还原

【Abstract】 The percentage of active individuals in a population of 388 leafhoppers (Nephotettixcincticeps), the vector transmitting the rice dwarf disease as determined by ELISA,agreed to the extent of 92.7% with the value obtained by biological assay. Thisrepresents an improvement over the 86% obtained two years ago in this laboratoryemploying the passive hemagglutination technique.更多还原