【摘要】 本文报道从人工贫血的兔与鸡的网织红细胞中提取珠蛋白mRNA,在麦胚无细胞体系中测定其蛋白翻译活力,与对照相比,兔、鸡珠蛋白mRNA促进¹⁴C-亮氨酸参入新生蛋白质的活力分别达到32倍和10倍,所翻译的蛋白产物在聚丙烯酰胺凝胶上的行为与天然珠蛋白一致。上述mRNA在AMV反转录酶和DNA聚合酶的作用下,分别合成了单链和双链的cDNA及mRNA-cDNA杂交链,凝胶电泳分析其长度分别为500～700,550～800及500～800碱基对,550～800碱基对大小的双链cDNA约占总合成量的30%。更多还原

【Abstract】 Globin mRNAs have been extracted from the reticulocytes of the rabbit andchicken, and their translation activities were measured in the wheat embryo cell-freesystem. In comparison with the control, rabbit and chicken globin mRNA, respec-tively, gave a 32-fold and 10-fold stimulation of ¹⁴C-leucine incorporation. The tran-slation products of these mRNAs co-migrated with authentic globin upon electropho-resis on SDS-polyacrylamide gel. In addition, single-and double-stranded globincDNA and the mRNA-cDNA hybrid strand have been synthesized from the mRNAsmentioned above with AMV reverse transcriptase and DNA polymerase 1. Analyzedby gel-electrophoresis the lengths of these cDNA fragments were 500～700, 550～800and 500～800 bp, respectively, and in the ds-cDNA synthesized, fragments with550～800 bp represent about 30% of the total cDNA.更多还原