【摘要】 <正> 前言结缔组织的染色方法如:Masson’s 染色法、Mallory’s 染色法、Heidenbain’sAzan 染色法、Maximow’s 染色法及 Crosso’s 染色法,常用于结缔组织切片的染色。用于疏松结缔组织铺片染色者,有铁苏木精染色法、锂卡红染色法、间苯二酚复红染色法,国内有杨振欧台盘兰注射染色法,另外还有专门显示某种细胞,如肥大细胞或浆细胞的染色法,显示某种纤维,如弹性纤维及胶元纤维的染色法等。上述染色法,均能不同程度地显示出结缔组织的染色特性,名有优点,但对疏松结缔组织的名种细胞及纤维更多还原

【Abstract】 The O.H.M.E staining method is used for demonstration and identifi- cation of cells and fibers of loose connective tissue.This staining techni- que has a special advantage of selecting stains for loose connective tissue. The method is as follows. 1、Take subcutaneous loose connective tissue out of animal body,then spread slightly a bits of tissue with dissecting needles on a slid.1t is im- portant to protect entirety of cells and not to tear it into pieces,therefore the manual spread out technique is the most essential action for to ensure the staining result. 2、Fixation should be in methyl alcohol for 5 minutes. 3、Rinse in 70 per cent alcohol for 15minutes, 4、Stain in Taenzer—Unna″s Orcein staining solution for 6—12hours at room temperature (or 30—60minutes at39℃). 5.Rinse in distilled water to remove excess stain. 6、Rinse in70 per cent alcohol for 2minutes 7、Stain in Ehrlich’s alum haematoxylin staining solution for 1 hour, 8、Rinse in distilled water.The cytoplasm want to stain grey,and examine under the microscope. 9、Decolorize in acid alcohol (70 per cent of 100mlalcohol add 0.25ml of HCl) to remove excess blue stain for 2——3 seconds. 10、Wash in tap—watar for 30 minutes until sections are blue. 11、Rinse in distilled water for10 minutes. 12、Incubate in appropriate Sorensen’s phosphote buffer(pH6.98—7.2) for 30 minutes. 13、Stain in M.E staining solution for 4hours or prolongation. Special reagent required: ①、stock stains. Solution M.——1:100 aqueous solution of methylene blue. Solution E.—1:100 aqueous solution of eosin. ②Staining Solution.To 100ml of phosphote buffer (pH6.98—7.2), Changing its pH from low up to high values in accordance with the require- ments of cytoplasmic colours from red to blue.add 35ml of Solution M. then add 45ml of solution E.The stain must be prepared before use and prevent it form precipitation. 14、Rinse in distilled water to remove excess stain.Examine under the microscope.If the section is excess stain,it should be treated with ba- sic absolute alcohol. 15、Diferentiate in basic absolute alcohol.(to100ml of absolute alcohol add 2—3drop of 40% sodium hydroxde) for a few seconds. 16、Dry in air,after 2hours mount in Canada balsam (or dehydrate rapidly with acetone.clear in xylol and mount in neutral mounting me- dium). 17、Result: elastic fibres——purple-brown. collagen fibres——salmon nuclei——deep blue——purple. cytoplasm——bluish—red. basophil granules——blue—black acidphil granulesl and blood cell——red, ground substance——pink更多还原