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牛白血病免疫扩散试验与临床血液检查结果对比分析
AN ANALYTICAL COMPARISON OF THE HEMATOLOGICAL EXAMINATION AND THE IMMUNODIFFUSION TEST FOR BOVINE LEUKOSIS
【摘要】 对六个牛群的1134头黑白花乳牛进行了牛白血病琼脂扩散及临床血液学检查。在无 BLV 感染及低感染牛群中,按南农检索表判定的血液检验阳性率分别为0.56%和0%;在高感染牛群中,琼扩试验阳性牛的血液检验阳性率均不超过30%,而且多数牛群远低于30%。琼扩试验阳性牛的血液检验阳性率(10.53%)高于琼扩试验阴性牛(2.73%),血液检验阳性牛的琼扩试验阳性率(70.77%)高于血液检验可疑牛(49.44%),而后者又高于血液检验阴性牛(35.41%),表明血液检验与琼扩试验结果存在明显的正相关。南农检索表判定的血液检验假阳性率(2.73%)低于 Bendixen检索表(10.62%),而其判定为阳性的牛只中琼扩试验结果为阳性的符合率(70.77%)又高于 Bendixen 检索表(58.89%),说明南农检索表更适合于我国南京和镇江地区作为黑白花乳牛 PL 的血液检查判定标准。
【Abstract】 An agar gel immunodiffusion(ID)test for detecting antibodies to bovineleukemic virus(BLV)and a hematological examination were carried out on 1134head of cattle in 6 herds.Measured by the Nanjing Agricultural College Key(NAC Key),the hematologieally positive percentages for persistent lymphocytosisin the BLV-free and the low infected herds were 0.56% and 0% respectively.Those of the ID reactors in the heavily infected herds were not more than30%,and in most cases they were far less.The percentage of hematologicallypositive animals in the ID reactors(10.53%)was higher than that in the IDtest negative animals(2.73%).The frequency of BLV infection among thehematologically positive cattle(70.77%)was higher than that of the hema-tologically suspected ones(49.44%),and the latter was higher than that of thehematologically negative animals(35.41%).These data suggest that the IDtest correlated closely with the hematological examination.Among the resultsevaluated by the NAC key,the false positive rate(2.73%)was considerablylower and the frequency of reactors in the ID test among the cattle withpersistent lymphocytosis(70.77%)was apparently higher than those evaluatedby Bendixen’s Key(10.62% and 58.89% respectively).These data indicatethat the NAC Key which was established natively from the normal lymphocytevalue is more applicable to the Nanjing and Zhenjiang Prefecture,JiangsuProvince.
- 【文献出处】 南京农业大学学报 ,Journal of Nanjing Agricultural University , 编辑部邮箱 ,1983年01期
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