【摘要】 本文介绍采用十二烷基硫酸钠(SDS)裂介赣科75—2甲型流感病毒颗粒得到仍具凝聚性的血凝素分子;在SDS条件下进行放射性碘化而后用SDS—醋纤膜电泳分离提纯标记的HA,建立了定量测定HA的放射火箭电泳自显影术。实验表明SDS环境对HA放射性碘化作用及标记HA的抗原性均无不良影响。此法可用于1—40HAu/ml的HA定量测定。更多还原

【Abstract】 This paper will describe another way to obtain influenza virus HA by sodium dodecyl sulphate(SDS) disruption.The HA molecules still have agglutination properties, ond under the condition of SDS,this HA can be radioiodinated and purified by electrophoresis on cellulose acetate strips. The radioiodination and the antigenicity of the HA do not affected by the SDS. A rocket-elec-trophoresis-autoradiography was also found. The HA from 1-40 HAU/ml can be quantitativeey measure by thismethod.更多还原