【摘要】 为了研究大白鼠视区胼胝神经元的分布及其特点,我们用12只成年大白鼠作了 HRP逆行追踪实验。一侧视皮层注射 HRP(30～50%,DAB 棕色反应);另一侧观察标记的神经元,结果如下:1.当注射位置离大脑中缝3.6 mm 或更近时,在对侧视皮层未观察到或偶尔观察到极少的标记神经元;当注射位置距大脑中缝4～6 mm 时,在对侧视皮层观察到大量标记神经元。2.在17～18a 区交界附近标记神经元最多。18a 区有较多的标记神经元,呈片状分布。17区内部较少。3.标记的神经元与注射部位之间有一定的对应关系:注射部位外移,标记神经元的区域也随之外移。4.标记神经元分布于皮层的Ⅱ～Ⅵ层,17区以Ⅳ、Ⅴ层较多,18a 区以Ⅲ、Ⅳ、Ⅴ层较多。5.部分标记神经元标记较浅,在暗视野下只能看出细胞体的轮廓。胞体直径8～10μm,个别可达16μm。标记清晰的主要是锥状神经元。其顶树突较长,指向皮层表面。基树突的分支清晰,胞体直径为10～20μm。此外还观察到多极和双极的神经元,胞体直径为10～20μm;颗粒形神经元,胞体直径在10μm 以下。将本实验结果与以往诸研究者的工作做了比较,并就与电生理研究结果的对应关系作了讨论。更多还原

【Abstract】 In order to investigate the distribution and other features of callosal neurons inthe visual areas,12 adult albino rats were used in a series of experiments,in whichHRP(30～50%,DAB brown reaction)was injected into one hemisphere and theretrograde labelled cells were observed in the other hemisphere.1.Hardly any labelled neuron was found in contralateral visual cortices whenthe injections were made at sites within 3.6 mm from the midline of the brain.However,when the injections were made at sites 4～6 mm from the midline,manylabelled neurons could be observed.2.Generally,most labelled cells aggregated near the border between area 17 and18a.In area 18a numerous cells were labelled,which distributed in the form ofincontinuous patches.In the proper of area 17,however,less labelled cells werefound.3.A gross topographical correlation was observed between locations of injectionand labelling on the two hemispheres.4.The labelled cells were found in all cortical layers except layer Ⅰ.In area 17they were mainly in layers Ⅳ and Ⅴ,while in area 18 a they were found mainly inlayers Ⅲ,Ⅳ and Ⅴ.5.Some callosal neurons were lightly labelled.For these cells,only the outlinesof the cell body could be seen even under dark-field microscope.The diameters ofthese cells were about 8～10μm.A few cells were as large as 16μm in diameter.Other labelled neurons could be seen with both dark-field and bright-field illumina-tions.They were identified as pyramidal,multipolar bipolar and granular cells accordingto the shape of the cell bodies and the pattern of dendrites.The majority of labelledcells were pyramidal cells,with a long apical dendrite to the pia surface,10～20μmin diameter.Other ceils were 10～20μm for multipolar cells,and less than 10μm forgranular cells.Comparisons were made between the above results and the physiological studies.更多还原