【摘要】 本文报道了醋酸棉酚对雄猴、大鼠和豚鼠腓肠肌超微结构的影响。雄性恒河猴按4毫克/公斤/日服棉酚两年,其腓肠肌超微结构基本保持正常。但个别动物腓肠肌肌细胞核出现固缩和核膜分离。成年 Wistar 雄性大鼠服抗生育剂量棉酚(20、30毫克/公斤/日×30)时,除光滑内质网轻度扩张及核膜分离外,腓肠肌超微结构与对照比较无明显变化。豚鼠实验亦得到类似结果。大鼠服棉酚剂量加大到(60毫克/公斤/日×37)时,虽然大部分肌原纤维仍保持正常的周期性结构,可见递次重复的明带和暗带。但某些肌原纤维失去正常纹理,有的Ⅰ带明显加宽,还有少数肌原纤维开始断裂、溶解,而线粒体未见明显病变。当大鼠服药时间延长至55日时,上述病变更加明显。线粒体细长,排列紊乱,同时更多的肌原纤维出现萎缩和断裂,表现为原纤维型坏死。豚鼠口服棉酚(60毫克/公斤/日×25)时,肌原纤维变化不甚明显,而部分线粒体出现不同程度的肿胀变性,损伤严重的线粒体嵴断裂溶解,表现为线粒体型坏死。本文初步分析和讨论了以上结果与低钾性肌无力的关系。更多还原

【Abstract】 Electron microscopic changes about the effect of gossypol acetic acid ongastrocnemius muscles of male monkeys,rats and guinea pigs were studied.The resultsare as follows:The ultrastructure of gastrocnemius muscles of adult male Rhesus monkeys afteradministration of gossypol orally with a daily dose of 4mg/kg for 2 years showedapparently normal in appearance,except some nuclear condensation and dissociationof nuclear membrance seen in individual animal.After oral aministration of gossypol in adult male Wistar rats with an antifertilitydosage,i.e 20mg or 30mg/kg/day for 30 days,no obvious difference was seen betweenthe experimented and control groups.Except mild distension of the smooth endoplasmicreticulum and dissociation of nuclear membrane sometimes seen in some of theexperiment animals.Similar results was obtained in the male guinea pigs.After a heavy dose(60mg/kg/day)of gossypol given to rats for 37 days,mostof the myofibrils still keep their normal periodic structures,each fiber is maintainingtheir transversly arranged pattern of dark A-bands and light I-bands alternativelyalong the whole length of the fiber.But some myofibrils had lost their normal striation.The width of the I-band increased and the breaking down or lysis precessesin few or even an individual myofibril had begun.There were no obvious pathologicalchanges seen in the mitochondria.Persistent oral administration of gossypol in ratewith a dosage of(60mg/kg/day)for 55 days,the pathological changes became moreobvious.The mitochondria became disordered in arrangement.Concomitant with thesschanges,the myofibrils appeared dystropic and fragmented.It indicated the presenceof myofibrillar degeneration.If the daily dose are increased to 60mg/kg for 25 days in guinea pigs.Theremight be no significant pathological changes in the myofibrils in them.Nevertheless,some mitochondria were swollen in various extents and the cristae became fragmentedor dissolved,which was known as mitochondrial necrosis.The relationship between hypokalemic paralysis and the present results inducedby toxic effect of gossypol were analysed and discussed.更多还原