【摘要】 用30只成年豚鼠的腹腔神经节在恒冷箱内制成连续切片,先按 De La Torre 乙醛酸法诱发荧光,对 SIF 细胞进行观察定位,随即在同一张切片上进行酶及其它组织化学反应。在所使用的反应条件下,与主神经元对比,SIF 细胞具有较强的 Mg⁺⁺激活的三磷酸腺苷酶,较弱的单胺氧化酶、乳酸脱氢酶及非特异性酯酶活性以及完全呈阴性反应的乙酰胆碱酯酶活性。用同时偶联法显示出SIF 细胞的酸性磷酸酶活性较弱,用铅法在大部分 SIF 细胞得到同样的结果。SIF 细胞质内脂类和核酸的含量均较主神经元少。此外,在荧光显微镜下可见到 SIF 细胞成团分布于血管周围,少数SIF 细胞发出长达100微米的细长突起。SIF 细胞的这些组织化学特性和形态特点表明它们可能以不同于主神经元的方式行使功能。内分泌或旁分泌可能是它们行使功能的主要方式。更多还原

【Abstract】 Serial cryostat sections of the celiac ganglion were prepared from 30 guinea pigs.The process of De Da Torre’s glyoxylic acid method and different histochmical rea-ctions were performed consecutively on the same section.Under the conditions employ-ed in this experiment,it was shown that the SIF cells,in contrast to the principalneurons,exhibited a more intense reation of adenosine triphosphatase,and a lessintense reaction of monoamine oxidase,lactic dehydrogenase and nonspecific esterase,and a negative reaction of alkaline phosphatase and acetylcholinesterase.The acidphosphatase reaction of most of SIF cells was weak as demonstrated with both theGomori’s lead method and the simultaneously coupling method.The SIF cells showedless lipids,nucleic acids and about the same content in tyrosin as compared with theprincipal neurons.These histochemical properties of SIF cells,as well as their morphological charac-teristics of grouping in clusters and surrounding blood vessels,implicated that theyfunction in a different way from that of principal neurons,and probably as endocrineand/or paracrine cells in modulating nervous transmission in the celiac ganglion.更多还原