【摘要】 作者用组织化学和电泳法,对5例成人来丸在微波照射前、后不同时期的精子乳酸脱氢酶-X进行了研究。结果表明微波照射后精子的乳酸脱氨酶-X活性,在一定时期下降,亦能恢复正常。并对微波影响精子乳酸脱氢酶-X的机理进行了讨论。更多还原

【Abstract】 Lactate dehydrogenase-X (LDH-X)is a specific enzyme of the adult ma-ture spermatozoa. The "band-X" ap-peared in a major area of LDH acti-vity between isozyme 3 and 4 onLDH electrophoretic pattern. Thedesignated LDH "band-X" was seenonly in the postpubertal testes. Thefunctional and metabolic significanceof the LDH-X in spermatozoa is un-known. This might be an index ofmale fertility [3]. Some authors sug-gested that LDH-X represents themost important isozyme for lactatemetabolism and even glycolysis in thehuman spermatozoa [7]. Becausethere are’nt any reports about the in-fluence of testes microwave irradia-tion on this new LDH, espeicially itshistochemical or electrophoretic studies,or about the relationship between thechange of this enzymatic activity andclinical contraception, more researchneeds to be done on this enzyme. In this paper, the authors observedthe LDH-X of human spermatozoa onthe histochemical smears and agarosegel electrophoretic patterns in 5 casesbefore and after microwave irradiationat different stages (i. e. 3 days, 12-15 days, 35-45 days, 72-88 days, 98-102 days after irradiation). It wasshown that LDH-X was deep bluepurple in color in the neck and middlepiece of the spermatozoa on the histo-chemical smears. On the LDH iso-zyme electrophoretic pattern, LDH-Xappears as a blue purple band whichis intermediate between LDH-3 andLDH-4. During the 35-45 days aftermicrowave irradiation of adult humantestes, the average percentage of LDH-X activity in 5 cases sharply de-creased as compared with the enzym-atic activity before microwave irra-diation, the average percentage hadan apparent difference (P<0.01). Itwas also found that LDH-X activityof mature spermatozoa returned to itsnormal level 72-102 days after micro-wave irradiation. The average percen-tage of the 5 cases showed no appar-ent difference from that before irra-diation (P>0.05). These phenomenashow that microwave may influenceLDH-X of the spermatozoa and theenzymatic activity may return to thenormal level at a certain. time aftermicrowave irradiation. Therefore, ifthe testes are irradiated by microwaveonce a month, LDH-X will be main-tained at a lower level. The lowerlevel of LDH-X may lead to the de-crease in the protein synthesis andthe motility of spermatozoa. This mayexplain the effective result producedin decreasing,fertility in clinical con-traception. The possible mechanism ofthe influence of microwave on LDH-Xof spermatozoa was also discussed.更多还原