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天花粉蛋白的化学 Ⅲ.溴化氰降解片段CBa的氨基酸顺序
CHEMISTRY OF TRICHOSANTHIN Ⅲ. AMINO AOID SEQUENCE OF CNBr DEGRADATION FRAGMENT CBa
【摘要】 <正> 前文已初步报道了天花粉蛋白的理化性质和N-端的部分氨基酸顺序.结晶天花粉蛋白经琥珀酸酐处理所得的琥珀酰天花粉蛋白,在88%甲酸中经溴化氰降解后的产物通过Sephadex G-75凝胶柱层析,可分得6个峰(见图1).
【Abstract】 The results from the study on the separation, purification, amino acid composition and amino acid sequence of CBa, one of the four CNBr degradation fragments of crystalline trichosanthin, are presented. Its amino acid composition is: Asp3, Thr2, Ser2, Hse1, Glu2, Gly2, Ala6, Val1, Tyr3, Phe3, Lys2, Arg1.The sequence of the CBa is Gly-Tyr-Arg-Ala-Gly-Asp-Thr-Ser-Tyr-Phe-Phe-Asn-Glu-Ala-Ser-Ala-Thr-Glu-Ala-Ala-Lys-Tyr-Val-Phe-Lys-Asp-Ala-Hse.
- 【文献出处】 化学学报 ,Acta Chimica Sinica , 编辑部邮箱 ,1983年12期
- 【被引频次】2
- 【下载频次】62