【摘要】 研究了两个低聚核糖核苷酸的3′-端磷酸化方法.以3′-端带磷酸单酯的低聚核苷酸为供体,用T₄ RNA连接酶将AUUC,CGGA,CUCGUCCA和CCAp等按低的供受体摩尔配比（1∶1.1至1∶2）,以87～90％的连接率合成了相应于酵母丙氨酸转移核糖核酸3′-端53～76核苷酸顺序的十九核苷酸AUUCCGGACUCGUCCACCAp.更多还原

【Abstract】 Two new 3’-phosphorylation procedures of oligoribonucleotide have been worked out. By means of using donor with a 3’-terminal phosphate group, under low ratio of donor/acceptor （1:1.1 to 1:2）, nonadecanucleotide AUUCCGGACUCGUCCACCAp, which corresponds to the 3’-terrninal nucleotides 58～76 of yeast alanine tRNA, has been synthesized in good yields （87～90%） through ligation by T₄ RNA ligase of four component fragments: AUUC, CGGA, CUCGUCCA and CCAp.更多还原