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5′-核苷二磷酸合成方法的改进及产物的分析

An improved method for the synthesis of nucleotides-5’-diphosphate and the analysis of the products

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【作者】费云标；宗建超；曾伟强；陈素明；李立朴；袁玉秀；邓金风；牟惠传；胡乃璧；

【Author】 Fei Yunbiao (1,6), Zong Jianchao (2), Zeng Weiqiang (1), Chen Suming (3), Li Lipu (3), Yuan Yuxiu (4), Deng Jinfeng (4), Mou Huichuan (5) and Hu Naibi(1),(1) Institute of Genetics, Academia Sinica, Beijing.(2) Department of Chemistry, Nankai University, Tianjin.(3) Institute of Chemistry, Academia Sinica, Beijing(4) Weijing (Sodium Glutamate) Manufactory, Tianjin.(5) Biochemical Medicine Manufactory, Tianjin.(6) Present address: Institute of Developmental Biology, Academia Sinica, Beijing.

【机构】中国科学院遗传研究所；南开大学化学系；中国科学院化学研究所；天津味精厂；天津市生物化学制药厂；中国科学院遗传研究所北京中国科学院发育生物学研究所北京；天津；北京；

【摘要】 <正> 5’-核苷二磷酸是一种重要的生化试剂。在其通常的化学合成中需要用离子交换柱分离纯化,操作较为繁杂,产率也不高。本文介绍的是通过升温直接从反应液中沉淀析出核苷二磷酸盐的简易和有效的方法及有关产物的分析研究。更多还原

【Abstract】 Nucleotides-5’-diphosphate were prepared by increasing the reaction temperature. The mixture containing pyridine, tributylaminephosphate and nucleotides-5’-phosphate-morpholine was heated to 50 to 65°G for about two days and nucleotides-5’-diphosphate separated out from the reaction liquid. Nuclear magnetic resonance and paper chromatographic analysis of the product showed that pure Li₃CDP could be prepared so ong as it was treated with LiOH and poly I:C could be prepared successfully with the product.更多还原

【关键词】核苷二磷酸；离子交换柱；生化试剂；反应液；分离纯化；图谱分析；沉淀析出；丙酮沉淀；三重峰；环己基；

【文献出处】化学试剂 ,Chemical Reagents , 编辑部邮箱 ,1983年03期

【被引频次】3
【下载频次】93

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