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电极法测定血清蛋白质

DETERMINATION OF SERUM PROTEIN BY ELECTRODE

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【作者】 王建华; 黄秩厚; 何福清;

【Author】 Wang Jianhua;Huang Zhihou;He Fuqing Department of Medicine,The Second Affiliated Hospital,Huna Medical College

【机构】 湖南医学院第二附属医院内科; 湖南医学院第二附属医院内科;

【摘要】 本文介绍了用氨气敏电极,以样品加入法测定血清中的蛋白质,并对方法误差进行了考察。本法与凯氏定氮法的测定结果无明显差异(P>0.05),重现性良好(C.V.0.52%),平均回收率为99.49%,方法简便、快速。

【Abstract】 A China-made ammonia selective electrode was used for the determination ofserum protein by means of the sample-added method.We have investigated theaccuracy of this method.The results of this method and that of Kjeldahl nitrogonanalysis were compared,Statistical analysis showed that there was no significantdifference between these two methods(P>0.05).The replication was good(C. V.0.52%).The average recovery rate of this method was 99.49%.

【关键词】 电极; 血清蛋白质;
【Key words】 electrodes serum proteins;
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