【摘要】 烙铁头Trimeresurus Mucrosquamatus蛇毒（TMV）可引起人和多种动物（狗、家兔、豚鼠）血小板的活化,发生聚集。血小板聚集强度与加入TMV的量有关。豚鼠血小板对TMV最为敏感,TMV引起豚鼠、家兔、狗和人的血小板聚集的最低剂量分别为0.6、12.5、2.0、2.0μg/ml。EDTA抑制而肝素不影响TMV对血小板的聚集作用。TMV的血小板聚集反应伴有5羟色胺的释放,引起家兔血小板5羟色胺最大释放（72％）的TMV剂量为100μg/ml。TMV还可以诱导血小板血栓恶烷A₂的形成。阿斯匹林能阻断TMV诱导的血栓恶烷A₂的生成,但并不抑制TMV引起的血小板聚集反应,提示TMV可能通过不依赖于血栓恶烷A₂的途径活化。初步结果表明TMV是研究血小板生理机制的有用工具。更多还原

【Abstract】 Trimeresurus Mucrosquamatus venom (TMV) induced the concentration-dependent aggregation of platelets of different species. The threshold concentrations of TMV inducing the platelet aggregation of human, rabbit, dog and Guinea pig were 2.0, 12.5, 2.0 and 0.6ug/ml respectively. EDTA interfered with the platelet aggregation by TMV, but heparin did not prevent the action of TMV. The platelet aggregation induced by TMV was accompanied by the release of serotonin (5-HT) from the challenged platelets. The concentration of TMV which induced the maxium release of 5-HT (72%) from rabbit platelets was 100ug/ml. The TMV also induced the formation of Thromboxane A2 (TXA2) of platelets. The inhibition of cyclo-oxygenase with aspirin, which reduced and abolished the formation of TXA2, but didnot suppress platelt aggregation induced by TMV. Thus, thromboxane-fndependent mechanisms should account for platelet activation with TMV. Our preliminary results shown that TMV is a good tool for studying the activation of platelets.更多还原