【摘要】 <正> 东北大黑鳃金龟(Holotrichia diomphalia Bates)是黑龙江省蛴螬优势种,为害严重,在旱田区、林区和园林苗圃中都有发生。近年来,在试验应用乳状菌防治蛴螬的探索中,因引进日本金龟(虫甲)芽孢杆菌(Bacillus popilliae var popilliae)对东北大黑鳃金龟不敏感,转而开始注意开发利用当地的乳状菌资源。在黑龙江省巴彦县龙泉林场,1978年秋、1982年春从自然感染乳状病的东北大黑鳃金龟幼虫中分离到A型和B型乳状芽孢杆菌各一株。四年来,对A型乳状芽孢杆菌进行了寄主范围、活体培养和离体培养、分类鉴定以及田间应用等方面的研究。致病力测定,该菌株对东北大黑鳃金龟的感病率高达81.8％,田间防治试验也取得了显著效果。更多还原

【Abstract】 A strain was isolated from Holotrichia diomphalia larve naturally infecting milky disease on the Bayan County Longquan Tree Farm of Heilongjiang Province. Through identifying, we have found that there are obvious differences between the strain and the other four described varieties on morphology, peros hostrange and some cultural characteristics. Therefore we consider that the strain is a new variety and named Bacillus popilliae var. holotrichia. In vitro culture, the strain grows on JV, JVB mediums. The tests of using the variety for scarabaei beetles cohtrol in nursery and farmland for four years indicate that the efficiency lsmarked.更多还原