【摘要】 <正> 激光微束照射技术是一种研究细胞的新方法,它对于研究细胞内各种结构的机能及细胞分化具有重要意义。我们曾报道了应用红宝石激光微束对体外培养癌细胞分裂期结构的研究。核仁是间期细胞的重要结构,它在细胞的蛋白质合成过程中起着极其重要的作用;一般认为生长迅速、代谢旺盛的细胞具有较大的核仁。对于核仁的研究已有不少报道,但对其机能的细节尚未揭示清楚。Berns等应用激光微束研究了核仁的机能及形成。本文应用红宝石激光微束照射体外培养癌细胞的核仁,观察核仁对癌细胞的生长及其在细胞周期不同阶段中的作用。更多还原

【Abstract】 We studied the relationship between the nucleolus and cell growth by laser microbeam-irradiation. A ruby laser microbeam was used to irradiate the nucleoli of Hela cells in vitro.The cells were not sensitized by treatment with a vital dye before laser microbeam-irradiation. Phase contrast microscope and fluorescence microscope examination of the irradiated nucleoli demonstrated that the primary site of damage was in the nucleolar material. Slight damage to the nucleoli caused the cells to grow slowly but cell growth became normal after 24 hours whereas severe damage to the nucleoli led to cell death. These results indicate that the nucleolus plays an important role in cell growth. (P 1 )更多还原