【摘要】 我们对SPRIA夹心法检测血清中抗-HBs的影响因素进行了探讨。我们认为在包被HBsAg的固相载体中加入待检血清样品,45℃孵育1～1.5小时,加入标记抗原,45℃孵育1.5～2小时,结合率最高。294份志愿献血者血清同时用SPRIA和PHA检测,SPRIA阳性97份,阳性率为32.99%;PHA阳性57份,阳性率为19.39%。SPRIA比PHA敏感10～20倍。更多还原

【Abstract】 The factors that might influence the data of SPRIA (sandwich) in determining anti-HBs in sera had been studied. We found that, add specimen to polystyrene bead with HBsAg incubate at 45℃ for 1～1.5 hrs, then add labeled HBsAg again incubate at 45℃ for 1.5～2 hrs would got the highest rate of combination.For comparison of SPRIA and PHA, 294 sera of voluntory donors were tested. 97 sara (32.99%)were found to have anti-HBs by SPRIA, while 57 sera (19.39%) were positive when using PHA.SPRIA was 10～20 fold more sensitive than PHA更多还原