【摘要】 <正> 1930年Tillet和Francis在炎症急性期病人血清中观察到有一种能特异性地与肺炎球菌细胞壁C多糖发生沉淀反应的蛋白质,后来被命名为C-反应蛋白(简称CRP)。 最初,CRP被认为是一种抗体,后来的实验证明它不是抗体,而是典型的非特异性的炎症急性期的反应性蛋白质。用放射免疫方法测定,CRP在正常动物血清中仅微量存在(平均为1340ng/ml血清),当动物患有组织损伤或炎症时,血清中CRP含量便成千倍地增加,几乎与组织损伤或炎症的程度成正比。当病理状况恢复正常时,CRP含量也下降。更多还原

【Abstract】 A procedure for purifying donkey c-reactive protein (CRP) by affinity chromato-graphy on phorsphoryl-choline-sepharose followed by DEAE-Sephadex A-25 chromatog-raphy and sephadex G-200 gel filtration is described. Purified donkey GRP was shown to be homogeneous by SDS polyacrylamide gel eletrophoreses.Molecular weight determination, amino acid analysis, as well as N-terminal group analysis of the subunit of donkey CRP indicated that donkey CRP is very much similar to human CRP,更多还原