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昆明地区秋季腹泻的病原学研究 Ⅱ.用酶联免疫吸附试验检测轮状病毒
Studies on the Etiology of Autumnal Acute Gastroenteritis in KunmingⅡ. Determination of rotavirus by an enzyme-linked immunosorbent assay
【摘要】 <正> 1980年我们曾证实轮状病毒(RV)是昆明地区婴幼儿急性胃肠炎的重要致病因子。随后用酶联免疫吸附试验(ELISA)检测RV。此法简便,快速,其敏感性不低于电镜。 材料和方法 1.临床标本:1980年10月至1981年1月因患急性胃肠炎住院病儿的粪便标本,经提纯浓缩,存
【Abstract】 A method for the detection of rotavirus by enzyme-linked immunosor-bent assay ( ELISA ) was presented. It is a specific, rapid, relatively simple and less expensive method. Thus it is suitable for determining rotavirus in clinical samples. In this study, the double antibody sandwich method was used. 43 fecal samples were examined. The results showed that ELISA is as sensitive as electron microscopic examination.
- 【文献出处】 中国医学科学院学报 ,Acta Academiae Medicinae Sinicae , 编辑部邮箱 ,1982年06期
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