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人白细胞干扰素基因的克隆化及其在大肠杆菌中的表达

Cloning of Human a Interferon cDNA from Umbilical Cord Leucocytes and Its Expression in E. coli

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【作者】侯云德；张智清；杨新科；吴淑华；李玉英；彭金枝；李应霞；杨崇泰；付秋林；胡裕文；赵海燕；王兰芝；段淑敏；苏小玲；张秀珍；张丽兰；史连永；刘小蕊；

【Author】 Hou Yunde, Zhang Zhiqing, Yang Xinke, Mu Shuhua, Li Yuying, Peng Jinzhi, Li Yingxia, Yang Chongtai, Fu Qiulin, Hu Yuwen, Zhao Haiyan, Wang Lanzhi, Duan Shumin, Su Xiaoling, Zhang Xiuzheng, Zhang Lilan, Shi Lianyong and Liu Xiaorui(Department of Antivirals, Institute of Virology,Beijing)

【机构】中国医学科学院病毒学研究所抗病毒治疗研究室；北京市输血站；中国医学科学院病毒学研究所抗病毒治疗研究室北京；北京；北京；

【摘要】以新城疫病毒F株诱生的人脐血白细胞内提取的12S聚(A)RNA制备dscDNA,以pBR322为载体,建立了人干扰素cDNA的无性繁殖系,后者为0.85kb,并在大肠杆菌中有明显表达,达1.3～18×10~4单位/升菌液。经血清学和理化鉴定,表达的多肽为人α干扰素。更多还原

【Abstract】 Double-stranded cDNA prepared from the 12 S fraction of poly ( A ) RNA from human umbilical cord leucocytes induced with F strain of Newcastle Disease Virus was cloned in E. coli ( HB 101 ) using pBR322 as a vector at pst-1 site. One of the obtained clones ( p8218 ) had a 850 base pair insert and could strongly hybridise to IFN-αDNA. E. coli transformed with this clone produced a protein with antiviral activity up to 1.3-18×104 international units per 1 1 of culture. This protein expressed in E. coli was characterized as human a interferon by neutralization test with monospecific anti-IFN-α or β globulins.更多还原

【关键词】克隆化；人脐血；理化鉴定；抗病毒；新城疫病毒；无性繁殖系； DNA； RNA；血清学；反转录酶；

【文献出处】中国医学科学院学报 ,Acta Academiae Medicinae Sinicae , 编辑部邮箱 ,1982年06期

【被引频次】41
【下载频次】322

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